Is it possible to use this fw on a different motherboard?

Without some coding, no.

Also not with a MKS Gen 1.4 for a Wanhao i3 V2.1? is there a chance to iniziall the rigth coding because i cant find any Firmware for the v2.1

The Wanhao i3 V2.1 does not have a DWIN DGUS LCD display. It is thus very different from the i3 Plus. I do not see the point to try to support this printer with ADVi3++.